Genetic manipulation techniques have enabled us to modify two major biomolecules, DNA and proteins, artificially. Nowadays, significant modifications of functions of proteins and cells have been achieved by complicated and large-scale modification of DNA/chromosomes based on advanced genetic manipulation technology, thus we can now apply the well modified functions in several applications such as molecular recognition, production of useful compounds, imaging, and so on.
This course covers the bases of protein engineering, genetic engineering, metabolic engineering, chromosome engineering and bioimaging, as well as advanced knowledge in these topics.
By the end of this course, students will be able to:
1) Understand the outline of genetic engineering and metabolic engineering, and acquire the advanced knowledge about genetic and metabolic manipulation of microbes for production of useful compounds.
2) Understand the outline of protein engineering, and acquire the advanced knowledge about protein thermostabilization, molecular evolutional methods, and antibody engineering.
3) Understand the outline of chromosome engineering, and acquire the advanced knowledge about artificial chromosomes, applications in generation of genetically modified organisms, and bioimaging.
Protein engineering, Genetic engineering, Metabolic engineering, Synthetic biology, Chromosome engineering, Bioimaging
|Intercultural skills||Communication skills||Specialist skills||Critical thinking skills||Practical and/or problem-solving skills|
Five instructors provide 2-4 classes with respect to the selected field in biomolecular engineering by using PowerPoint slides.
|Course schedule||Required learning|
|Class 1||Advanced techniques for Genetic Engineering||Explain the basis and advanced techniques for genetic engineering.|
|Class 2||Microbial metabolisms and overview of metabolic engineering.||Explain the properties of microbial metabolisms and notion and methodologies for metabolic engineering.|
|Class 3||Recent metabolic engineering of microbes for production of useful compounds||Understand and explain recent applications of microbial metabolic engineering aiming production of useful compounds.|
|Class 4||Artificial chromosomes and chromosome engineering||Understand genetic engineering using artificial chromosomes, and introduce up-to-date technology of chromosome engineering.|
|Class 5||Genetic/developmental engineering and genome editing to generate genetically modified organisms||Introduce up-to-date technology in genetic and developmental engineering.|
|Class 6||Bioimaging using genetic engineering techniques||Understand bioimaging techniques using genetically modified proteins, and introduce its applications in recent researches.|
|Class 7||Engineering of fluorescent protein indicators||Understand and explain the development of genetically-encoded biosensors based on fluorescent proteins.|
|Class 8||Engineering of bioluminescent indicators||Understand and explain the design and application of biosensors based on luciferases.|
|Class 9||Controlling cell signaling by engineered proteins||Understand and explain the biological techniques for manipulating cell function.|
|Class 10||Protein Engineering (1) Overview and Thermostabilization||Explain the notion of and the methodologies used for protein engineering, and thermostabilization as an important application.|
|Class 11||Protein Engineering (2) Rational design||Explain the basis and applications of rational design methods, as an important methodology of protein engineering.|
|Class 12||Protein Engineering (3) Molecular evolution||Explain the basis and applications of molecular evolution, as an important methodology of protein engineering.|
|Class 13||Protein Engineering (4) Antibody Engineering||Explain the basis and several applications of antibody engineering, one of most industrially important area of protein engineering.|
|Class 14||Photosynthesis and Carbon Metabolism||Explain the relationship of photosynthesis and carbon metabolism in photosynthetic organisms|
|Class 15||Plant Lipid Metabolism and Lipid Metabolic Engineering||Explain plant lipid metabolism and lipid metabolic engineering|
Handouts will be distributed at the beginning of class when necessary. The PowerPoint documents that are to be used in the class will be made available in advance via the OCW-i system, as possible.
Students will be assessed by reports indicated by each instructor (20% each).
Although there is no special requirement, students who take this course are required to have basic knowledge of biochemistry/molecular biology by studying the related subjects.
This lecture is given in English, but some supplementary explanation in Japanese may be introduced in the last part of the classes.