2024 LST Basic Laboratory and Exercise I D

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Academic unit or major
Undergraduate major in Life Science and Technology
Kitao Akio  Ishii Yoshitaka  Itoh Takehiko  Ueno Takafumi  Kamachi Toshiaki  Kobatake Eiry  Tokunaga Makio  Murakami Satoshi  Osada Toshiya  Nozawa Kayo  Mie Masayasu  Miyashita Eizo  Yamada Takuji  Asakura Noriyuki  Kondo Toru  Taguchi Hideki  Okada Ui  Tran Duy Phuoc  Denda Kimitoshi  Furuta Tadaomi  Niwa Tatsuya  Ito Hidehiro  Nishida Kei  Hayashi Nobuhiro  Wong Sing Ying  Matsunaga Tatsuya  Kamiya Mako  Shiraki Nobuaki  Fukui Toshiaki  Yasui Takao  Uriu Koichiro  Ajiri Taiga  Tanaka Hiroyuki 
Class Format
Exercise /Experiment    (Face-to-face)
Media-enhanced courses
Day/Period(Room No.)
Mon5-9(緑が丘3号館学生実験室)  Thr5-9(緑が丘3号館学生実験室)  
Course number
Academic year
Offered quarter
Syllabus updated
Lecture notes updated
Language used
Access Index

Course description and aims

This course aims at learning the experimental principles and methods of physical chemistry and related knowledge that are required to carry out bachelor thesis research.

Student learning outcomes

By the end of this course, students will be able to:
1) Conduct basic experiments required for bachelor thesis research.
2) Devise a troubleshooting method when the experiment fails.
3) Clearly note the purpose, results, and discussion of the experiment.


Physical chemistry

Competencies that will be developed

Specialist skills Intercultural skills Communication skills Critical thinking skills Practical and/or problem-solving skills

Class flow

The experiment will be conducted after explaining the experiment in about 30 minutes.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Basics and usage of spectrophotometer I  
Class 2 Basics and usage of spectrophotometer II
Class 3 Separation and purification of proteins I    
Class 4 Separation and purification of proteins II  
Class 5 Separation and purification of proteins III  
Class 6 Kinetic analysis of enzyme reaction I  
Class 7 Kinetic analysis of enzyme reaction II  
Class 8 Kinetic analysis of enzyme reaction III  
Class 9 Analysis of protein-ligand binding by equilibrium dialysis  
Class 10 Computer analysis of biomolecules I  
Class 11 Measurement of acid dissociation constant  
Class 12 Computer analysis of biomolecules II  
Class 13 Computer analysis of biomolecules II Quantification of diffusion coefficient and Avogadro number by Brownian motion measurement  
Class 14 Computer analysis of biomolecules III  

Out-of-Class Study Time (Preparation and Review)

To enhance effective learning, students are encouraged to spend a certain length of time outside of class on preparation and review (including for assignments), as specified by the Tokyo Institute of Technology Rules on Undergraduate Learning (東京工業大学学修規程) and the Tokyo Institute of Technology Rules on Graduate Learning (東京工業大学大学院学修規程), for each class.
They should do so by referring to textbooks and other course material.


Original text created by the the School of Life Science and Technology

Reference books, course materials, etc.

No reference book is specified.
Handouts will be distributed as needed.

Assessment criteria and methods

The grade is evaluated by the attitude toward the experiment, the degree of skill of the experiment operation technique, and the experiment report.

Related courses

  • LST.A222 : LST Basic Laboratory II
  • LST.A223 : LST Basic Laboratory III
  • LST.A224 : LST Basic Laboratory IV

Prerequisites (i.e., required knowledge, skills, courses, etc.)

No special requirements

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