2020 LST Basic Laboratory and Exercise IV D

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Academic unit or major
Undergraduate major in Life Science and Technology
Fukui Toshiaki  Yamaguchi Yuki  Wachi Masaaki  Aizawa Yasunori  Tagawa Yoh-Ichi  Nakatogawa Hitoshi  Yatsunami Rie  Kajikawa Masaki  Kondoh Shinae  Yamamoto Naoyuki  Kadonosono Tetsuya  Tachibana Kazunori  Iwasaki Hiroshi  Fujita Naonobu  Ueda Hiroshi  Imamura Sousuke  Kitaguchi Tetsuya  Wakabayashi Ken-Ichi  Kano Fumi  Iwai Noritaka  Okumura Eiichi  Orita Izumi  Kaneko Shinya  Sakamoto Satoshi  Tanaka Toshiaki  Iwatani Shun  Tsubouchi Hideo  Fukushima Toshiaki  Omuro Yuki  Kobayashi Yuki  Nomata Jiro  Yamamoto Junichi 
Course component(s)
Exercise /Experiment
Day/Period(Room No.)
Intensive 2-8(緑が丘3号館学生実験室)  
Course number
Academic year
Offered quarter
Syllabus updated
Lecture notes updated
Language used
Access Index

Course description and aims

This course aims at learning principles and methods for basic experiments in molecular biology, which are required for conducting research in the laboratory.

Student learning outcomes

By the end of this course, students will be able to:
1) Conduct basic molecular biological experiments required for research in the laboratory.
2) Solve problems when in trouble with basic molecular biological experiments.
3) Describe the aim, results, and discussion of molecular biological experiments clearly.


Molecular biology

Competencies that will be developed

Intercultural skills Communication skills Specialist skills Critical thinking skills Practical and/or problem-solving skills

Class flow

Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Cultivation of microorganisms 1 Experimental report.
Class 2 Cultivation of microorganisms 2 Experimental report.
Class 3 Transformation of Escherichia coli 1 Experimental report.
Class 4 Transformation of Escherichia coli 2 Experimental report.
Class 5 Transformation of Escherichia coli 3 Experimental report.
Class 6 Analysis of DNA topology and higher order structure 1 Experimental report.
Class 7 Analysis of DNA topology and higher order structure 2 Experimental report.
Class 8 Analysis of DNA topology and higher order structure 3 Experimental report.
Class 9 Analysis of mRNA expression 1 Experimental report.
Class 10 Analysis of mRNA expression 2 Experimental report.
Class 11 Analysis of mRNA expression 3 Experimental report.
Class 12 Analysis of gene mutation conferring resistance to antibiotics 1 Experimental report.
Class 13 Analysis of gene mutation conferring resistance to antibiotics 2 Experimental report.
Class 14 Analysis of gene mutation conferring resistance to antibiotics 3 Experimental report.


An original textbook edited by the School of Life Science and Technology

Reference books, course materials, etc.

Handouts will be distributed as necessary.

Assessment criteria and methods

Experimental training, report

Related courses

  • LST.A208 : Molecular Biology I
  • LST.A213 : Molecular Biology II

Prerequisites (i.e., required knowledge, skills, courses, etc.)




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