Cell engineering involves the characterization and manipulation of cell functions with their application in life science research and regenerative medicine.The couse will cover the base and aplicaitons of celll engineering, combined with molecular cell biology, developmental biology and genetic engineering to establish fundamental understanting of property-function relationships and manipulation of cell properties to alter, maintain,restore or improve cell functions. This course will also give an overview of milestones of in vivo genetic engineering and stem cell researchs and expose students to current topics of these fields, in which techniques for cell engineering, live-imaging, gene-targeting, and stem cells engineering will be highlighted.
By the end of this course, students will be able to:
1) Understand how to ulitize cell and cell engineering technology.
2) Understand the principles and applications of cell fusion
3) Understand the principles and applications of transfection.
4) Understand the principles and applications of bioimaging techniques.
5) Establishes a solid base of generation of generically modified animals.
6) Understand stem cell biology including ES and iPS cells.
7) Uderstand the principles and applicaiton of stem cell for regenerative medicine
Cell, Cell death, Proliferation, Aging, Immortalization, Cell fusion, gene expression, transfection, bioimaging, transgenic, gene-targeting stem cell, embryonic stem cell, iPS, regenerative medicine
|Intercultural skills||Communication skills||Specialist skills||Critical thinking skills||Practical and/or problem-solving skills|
In the first quater of the class, a summary of the previous lecture followed by the main points of the day’s lecture posed asquestions are given. Then, these main points are discussed in detail. Always check the required learning for each class and besure to complete them as part of preparation and review.
|Course schedule||Required learning|
|Class 1||Introduction to Cell Engineering 1||Understand aims of this course. Stucent will be introduced to cell engineering and regenerative medicine.|
|Class 2||Introduction to Cell Engineering 2||Understand history and fundamantals of cell engineering, cell sources and stem cells.|
|Class 3||Cell fusion||Understand principles of cell fusion and its application including production of a monoclonal antiboddy.|
|Class 4||Genetic manipulation of mammalian cells||Students will be introduced to genetic manipulation of mammalian cells including designs of expression vector and various techniques of transfection.|
|Class 5||Bioimaging||Understand principles and application of live imaging technology using genetically engineered fluorescent proteins.|
|Class 6||Introduction to stem cells||Students will be provided with an overview of embryogenesis and embryonic stem cell.|
|Class 7||Transgenesis||Understand principles and applications of generation of genetically engineered animals|
|Class 8||Principle of Gene-targeting||Understand principles of gene-targeted animals, including knock-out / knock-in mice.|
|Class 9||Application of gene-targeting techniques||Understand applications of conditional gene-targeting techniques of including Cre-loxP system, drug-induced gene egineering.|
|Class 10||Gene therapy||Understand principles and applicatinos of gene therapy.|
|Class 11||Stem cell biology and its appliaction||Students will be provided with an overview of pluripotent stem cells from embryonic stem cells to iPS cells.|
|Class 12||Approaches for developmental biology||Understand genetic engineering and reprograming of stem cells and iPS cells.|
|Class 13||in vitro differentiation of iPS cells||Understand base of cell differentiation and in vitro differentiation of iPS cells.|
|Class 14||Cell transplantation and regenerative medicine||Students will be provided with an overview of current stem cell applications in regenerative medicine.|
|Class 15||Modeling disease using iPS cells||Understand use of iPS and adult stem cells in modeling desease.|
None is required.
Molecular Biology of the Cell, 6th Edition (Bruce Alberts et al., Garland Science)
Biochemistry, 4th Edition (Donald Voet, Judith G. Voet, Wiley)
Assessment is based on the final exam (100%).
Knowledge of Biochemistry, Molecular Biology, Genetic Engineering is required.