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2017　Directed Laboratory Works in Biomolecular Engineering II

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Academic unit or major
Biomolecular Engineering
Instructor(s)
Academic Supervisor 
Class Format
    
Media-enhanced courses
Day/Period(Room No.)
Intensive ()  
Group
-
Course number
ZBE.B401
Credits
2
Academic year
2017
Offered quarter
3-4Q
Syllabus updated
2017/9/6
Lecture notes updated
-
Language used
Japanese
Access Index

Course description and aims

This is a laboratory course covering the following topics on organic chemistry, physical chemistry, biochemistry for students who are second-year in the department of biotechnology. The aims of the course are to develop their abilities in the experimental skills for biotechnology that are essential to become successful engineers and researchers. Furthermore, the course gives opportunity for safety training, processing data, and preparing technical reports.
Throughout the experiments, students will be endowed with teamwork, leadership, and communication skills.

Student learning outcomes

By the end of this course, students will be able to:
1. Understand the design of biohybrid catalysts and protein bioinformatics using a personal computer.
2. Understand the production and purification of proteins using an affinity tag, and time-resolved absorption spectroscopy of a transient species with a lifetime of 1 microsecond.
3. Understand the preparation and characterization of thermoresponsive polymers, and differentiation of beating cardiac cells from mouse embryonic stem cells. 4. Understand the synthesis of medicinally or biologically important compounds.

Keywords

catalyst, bioinformatics, affinity tag, thermosensitive polymer, embryonic stem cells, differentiation spectroscopy, organic synthesis

Competencies that will be developed

✔ Specialist skills Intercultural skills ✔ Communication skills ✔ Critical thinking skills ✔ Practical and/or problem-solving skills

Class flow

This course is held over two academic quarters. The written report must be submitted by a date specified. Students must read each part of the given experiment and prepare a procedure flow in advance to understand about the experiment and to ensure safety and smooth running.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Design of biohybrid catalysts #1 Learn the protein crystallization method and applications of protein crystals as solid materials.
Class 2 Design of biohybrid catalysts #2 Learn the protein crystallization method and applications of protein crystals as solid materials.
Class 3 Exercise for protein bioinformatics using a personal computer #1 Learn the application of various bioinformatics tools to proteins
Class 4 Exercise for protein bioinformatics using a personal computer #2 Learn the application of various bioinformatics tools to proteins
Class 5 Production and purification of proteins using an affinity tag #1 Explain production and purification of proteins using an affinity tag
Class 6 Production and purification of proteins using an affinity tag #2 Explain production and purification of proteins using an affinity tag
Class 7 Preparation and Characterization of Thermoresponsive Polymers #1 Prepare thermoresponsive polymers and understand thermodynamic properties of the polymer solution.
Class 8 Preparation and Characterization of Thermoresponsive Polymers #2 Prepare thermoresponsive polymers and understand thermodynamic properties of the polymer solution.properties of the polymer solution.
Class 9 Differentiation of beating cardiac cells from mouse embryonic stem cells #1 Explain differentiation of mouse embryonic stem cells to beating cardiac cells
Class 10 Differentiation of beating cardiac cells from mouse embryonic stem cells #2 Explain differentiation of mouse embryonic stem cells to beating cardiac cells
Class 11 Time-resolved absorption spectroscopy of a transient species with a lifetime of 1 microsecond #1 Training of the strict measurements of spectra by using spectrophotometer and fuluorophotometer
Class 12 Time-resolved absorption spectroscopy of a transient species with a lifetime of 1 microsecond #2 Training of the strict measurements of spectra by using spectrophotometer and fuluorophotometer Quantum chemical understanding of electronic transitions shown by the measured spectra.
Class 13 Synthesis of medicinally or biologically important compounds #1 Students will be able to: Carry out organic reactions for synthesizing medicines; including reaction design, practical synthesis, and analysis of purified products.
Class 14 Synthesis of medicinally or biologically important compounds #2 Students will be able to: Carry out organic reactions for synthesizing medicines; including reaction design, practical synthesis, and analysis of purified products.
Class 15 Synthesis of medicinally or biologically important compounds #3 Students will be able to: Carry out organic reactions for synthesizing medicines; including reaction design, practical synthesis, and analysis of purified products.

Textbook(s)

The experimental manuals are distributed in each lab.

Reference books, course materials, etc.

None

Assessment criteria and methods

Full attendance and completion of all experiments are compulsory.
Assessment is based on the quality of the written reports and on the status of submission thereof.
The instructor may fail a student if he/she repeatedly comes to class late or resubmits reports too often.

Related courses

  • Organic Chemistry I
  • CHM.D202 ： Organic Chemistry II
  • Physical Chemistry I
  • Physical Chemistry II
  • LST.A203 ： Biochemistry I
  • Biochemistry II

Prerequisites (i.e., required knowledge, skills, courses, etc.)

None

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