2019 Basic Bioscience Laboratory II

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Course description and aims

On the premise that the students have completed "Basic Bioscience Laboratory I", this second course aims to nurture the abilities to carry out experimental studies on their own by having them learn basic skills behind the more extended areas than those the first course covered.

Student learning outcomes

When all of the course menus are complete, students will have learned the concept of experimental error, extraction, chromatography, centrifugation, electrophoresis, and the qualitative analysis of organic compounds. Especially, they will develop the basic experimental skills in biochemistry, organic chemistry, and physical chemistry.


Life science experiment, biochemistry, organic chemistry, physical chemistry, separation

Competencies that will be developed

Intercultural skills Communication skills Specialist skills Critical thinking skills Practical and/or problem-solving skills
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Class flow

In the first 30 min, the contents of experiments will be detailed. Then, each group with 2 or 3 students will carry out a given experimental subject based on what they read in the text book beforehand and learned in the introduction lecture. Teaching assistants are ready to instruct the students when they are stuck during the experiments. When the assigned experiments are complete, the students must report it to a teaching assistant and finalize the subject by tidying up the bench and summarizing data. Some subjects may assign you to submitt the report at the day of its experiment.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Basic skills 1 Buffer preparation for biochemistry experiments
Class 2 Basic skills 2 Experimental errors, the water used for life science experiments
Class 3 Biochemistry 1 Separation of proteins by centrifugation
Class 4 Biochemistry 2 Separation of proteins by column chromatography
Class 5 Biochemistry 3 Preparation of electrophoresis gels
Class 6 Biochemistry 4 Separation of proteins by electrophoresis
Class 7 Organic chemistry 1 Thin-layer chromatography
Class 8 Organic chemistry 2 Separation of organic compounds by thin-layer chromatography
Class 9 Organic chemistry 3 Separation of organic compounds by liquid-liquid extraction
Class 10 Organic chemistry 4 Qualitative analysis of organic compounds
Class 11 Biochemistry 5 Kinetics of enzyme reactions
Class 12 Biochemistry 6 Extraction and classification of lipids
Class 13 Physical chemistry 3 Distribution factor
Class 14 Physical chemistry 4 Programming with BASIC
Class 15 Summary Reviewing the whole outcomes


The textbook of life science basic experiments 1 & 2, Department of Life Science, School of Bioscience and Biotechnology, Tokyo Institute of Technology (free hand out)

Reference books, course materials, etc.


Assessment criteria and methods

Experimental training (40%), report (60%)

Related courses

  • ZUW.A221 : Basic Bioscience Laboratory I

Prerequisites (i.e., required knowledge, skills, courses, etc.)

Basic Bioscience Laboratory I is compulsory.

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