On the premise that the students have completed "Basic Bioscience Laboratory I", this second course aims to nurture the abilities to carry out experimental studies on their own by having them learn basic skills behind the more extended areas than those the first course covered.
When all of the course menus are complete, students will have learned the concept of experimental error, extraction, chromatography, centrifugation, electrophoresis, and the qualitative analysis of organic compounds. Especially, they will develop the basic experimental skills in biochemistry, organic chemistry, and physical chemistry.
Life science experiment, biochemistry, organic chemistry, physical chemistry, separation
|Intercultural skills||Communication skills||Specialist skills||Critical thinking skills||Practical and/or problem-solving skills|
In the first 30 min, the contents of experiments will be detailed. Then, each group with 2 or 3 students will carry out a given experimental subject based on what they read in the text book beforehand and learned in the introduction lecture. Teaching assistants are ready to instruct the students when they are stuck during the experiments. When the assigned experiments are complete, the students must report it to a teaching assistant and finalize the subject by tidying up the bench and summarizing data. Some subjects may assign you to submitt the report at the day of its experiment.
|Course schedule||Required learning|
|Class 1||Basic skills 1||Buffer preparation for biochemistry experiments|
|Class 2||Basic skills 2||Experimental errors, the water used for life science experiments|
|Class 3||Biochemistry 1||Separation of proteins by centrifugation|
|Class 4||Biochemistry 2||Separation of proteins by column chromatography|
|Class 5||Biochemistry 3||Preparation of electrophoresis gels|
|Class 6||Biochemistry 4||Separation of proteins by electrophoresis|
|Class 7||Organic chemistry 1||Thin-layer chromatography|
|Class 8||Organic chemistry 2||Separation of organic compounds by thin-layer chromatography|
|Class 9||Organic chemistry 3||Separation of organic compounds by liquid-liquid extraction|
|Class 10||Organic chemistry 4||Qualitative analysis of organic compounds|
|Class 11||Biochemistry 5||Kinetics of enzyme reactions|
|Class 12||Biochemistry 6||Extraction and classification of lipids|
|Class 13||Physical chemistry 3||Distribution factor|
|Class 14||Physical chemistry 4||Programming with BASIC|
|Class 15||Summary||Reviewing the whole outcomes|
The textbook of life science basic experiments 1 & 2, Department of Life Science, School of Bioscience and Biotechnology, Tokyo Institute of Technology (free hand out)
Experimental training (40%), report (60%)
Basic Bioscience Laboratory I is compulsory.