2019 Basic Bioscience Laboratory I

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Course component(s)
Day/Period(Room No.)
Intensive (大岡山・すずかけ台の各実験担当研究室)  
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Syllabus updated
Lecture notes updated
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Course description and aims

1)Knowledge and management of wastes and hazardous materials will be detailed. 2) Principles and basic operation and maintenance methods of experimental apparatuses and instruments in life science will be demonstrated and practiced through appropriate experiments. Specifically, buffer preparation, pH measurement, weighing solids, pipetting liquids, and spectrometry are focused. 3) On the above fundamentals, some practical experiments and activities are experienced.

Student learning outcomes

When all of the course menus are complete, students will have learned how to prepare, safely do, and finalize the experiments, with the basic knowledge of using apparatuses and instruments. At the same time, they will be proficient in recording data properly in their own notes, rational thinking during experiments, and writing scientific reports. Especially, they will develop the basic experimental and operational skills in physical chemistry, molecular biology, biology, and bioinformatics.


Life science experiment, physical chemistry, molecular biology, biology, bioinformatics

Competencies that will be developed

Intercultural skills Communication skills Specialist skills Critical thinking skills Practical and/or problem-solving skills
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Class flow

In the first 30 min, the contents of experiments will be detailed. Then, each group with 2 or 3 students will carry out a given experimental subject based on what they read in the text book beforehand and learned in the introduction lecture. Teaching assistants are ready to instruct the students when they are stuck during the experiments. When the assigned experiments are complete, the students must report it to a teaching assistant and finalize the subject by tidying up the bench and summarizing data. Some subjects may assign you to submitt the report at the day of its experiment.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Orientation How to write the reports for life science experiments, how to dispose wastes, how to ensure safety, how to use pipettes and Pipetman.
Class 2 Basic skills 1 How to use pH meters
Class 3 Basic skills 2 How to use spectrometers
Class 4 Physical chemistry 1 Viscosity of liquid
Class 5 Physical chemistry 2 Measurement of diffusion coefficient
Class 6 Practical computer science 1 Computer literacy
Class 7 Practical computer science 2 Basics in bioinformatics
Class 8 Basic skills 3 Buffer preparation for molecular biology experiments
Class 9 Molecular biology 1 Transformation of colon bacilli
Class 10 Molecular biology 2 Extraction of plasmid DNAs from colon bacilli
Class 11 Molecular biology 3 Electrophoresis of plasmid DNAs
Class 12 Biology 1 Observation of vertebrate embryos development
Class 13 Biology 2 Cell cultivation
Class 14 Biology 3 Observation of first stage culture cell of chick embryo
Class 15 Summary Reviewing the whole outcomes


The textbook of life science basic experiments 1 & 2, Department of Life Science, School of Bioscience and Biotechnology, Tokyo Institute of Technology (free hand out)

Reference books, course materials, etc.


Assessment criteria and methods

Experimental training (40%), report (60%)

Related courses

  • ZUW.A222 : Basic Bioscience Laboratory II

Prerequisites (i.e., required knowledge, skills, courses, etc.)

Attendance in the first class (orientation) is compulsory.

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