１）Knowledge and management of wastes and hazardous materials will be detailed. 2) Principles and basic operation and maintenance methods of experimental apparatuses and instruments in life science will be demonstrated and practiced through appropriate experiments. Specifically, buffer preparation, pH measurement, weighing solids, pipetting liquids, and spectrometry are focused. 3) On the above fundamentals, some practical experiments and activities are experienced.
When all of the course menus are complete, students will have learned how to prepare, safely do, and finalize the experiments, with the basic knowledge of using apparatuses and instruments. At the same time, they will be proficient in recording data properly in their own notes, rational thinking during experiments, and writing scientific reports. Especially, they will develop the basic experimental and operational skills in physical chemistry, molecular biology, biology, and bioinformatics.
Life science experiment, physical chemistry, molecular biology, biology, bioinformatics
|Intercultural skills||Communication skills||Specialist skills||Critical thinking skills||Practical and/or problem-solving skills|
In the first 30 min, the contents of experiments will be detailed. Then, each group with 2 or 3 students will carry out a given experimental subject based on what they read in the text book beforehand and learned in the introduction lecture. Teaching assistants are ready to instruct the students when they are stuck during the experiments. When the assigned experiments are complete, the students must report it to a teaching assistant and finalize the subject by tidying up the bench and summarizing data. Some subjects may assign you to submitt the report at the day of its experiment.
|Course schedule||Required learning|
|Class 1||Orientation||How to write the reports for life science experiments, how to dispose wastes, how to ensure safety, how to use pipettes and Pipetman.|
|Class 2||Basic skills 1||How to use pH meters|
|Class 3||Basic skills 2||How to use spectrometers|
|Class 4||Physical chemistry 1||Viscosity of liquid|
|Class 5||Physical chemistry 2||Measurement of diffusion coefficient|
|Class 6||Practical computer science 1||Computer literacy|
|Class 7||Practical computer science 2||Basics in bioinformatics|
|Class 8||Basic skills 3||Buffer preparation for molecular biology experiments|
|Class 9||Molecular biology 1||Transformation of colon bacilli|
|Class 10||Molecular biology 2||Extraction of plasmid DNAs from colon bacilli|
|Class 11||Molecular biology 3||Electrophoresis of plasmid DNAs|
|Class 12||Biology 1||Observation of vertebrate embryos development|
|Class 13||Biology 2||Cell cultivation|
|Class 14||Biology 3||Observation of first stage culture cell of chick embryo|
|Class 15||Summary||Reviewing the whole outcomes|
The textbook of life science basic experiments 1 & 2, Department of Life Science, School of Bioscience and Biotechnology, Tokyo Institute of Technology (free hand out)
Experimental training (40%), report (60%)
Attendance in the first class (orientation) is compulsory.