What is the life? Why? To answer these questions, the aim of this course is to form a habit of considering using basic principles, essential models, and quantitative measures. Seeing the various workings of life and the cells through this physical way of thinking, not the physics with difficult numerical formula, interests and admirations of the physics can be enjoyed. Connecting physical chemistry learned so far with biochemistry, molecular biology, and biology to consider and discuss the "Why?" of concrete biological phenomena leads understanding of essence of life phenomena, and enhance the ability of scientific considering. Advanced materials on molecular mechanisms in life science will be discussed.
1) Overlooking of biological phenomena using number, space, and time as a quantitative measure.
2) On the basis of concrete examples, explaining that maximum of entropy of whole system and minimum of Gibbs energy of the system are basic principles conducting biological phenomena thermodynamically.
3) Learning theory of random walk, soft chain, and Bernoulli-Euler beam, and discussion about structures of biopolymers and cells.
4) Quantitative understanding of biomembrane and membrane proteins, and bioelectricity and action potential generated by the membrane.
5) On the basis of concrete examples, discussion about dynamics of reactions in the cells and biomolecular motor.
biopolymer, cell function, biomembrane, protein, bioelectricity, action potential, biomolecule motor, thermodynamics, transport, reaction dynamics, membrane transport
|Intercultural skills||Communication skills||Specialist skills||Critical thinking skills||Practical and/or problem-solving skills|
The lecture is to be done in order of the contents of the textbook. (Thus, students are encouraged to familiarize the expected contents of the textbook in advance and to review them after the class.) The lecture will be given in English.
|Course schedule||Required learning|
|Class 1||Why: Biology by the Numbers --- Application of physical and quantitative thinking to biological problems. (Yoshitaka Ishii)||Understand the importance of fundamental physical models and quantitative estimation of the biologycal system.|
|Class 2||What and Where: Construction Plans for Cells and Organisms --- Development of an intuitive feeling for size and scale to envision the biological processes. (Yoshitaka Ishii)||Understand the various units consisting biological system.|
|Class 3||When: Stopwatches at Many Scales --- Understanding different views of time in biological systems. (Yoshitaka Ishii)||Understand the different time scales in biological systems.|
|Class 4||Mechanical and chemical equilibrium in the living cell. (Nobuhiro Hayashi)||Understanding energy providing directions of the biological reactions.|
|Class 5||Entropy rules! (Nobuhiro Hayashi)||Understanding life phenomena through statistical mechanics.|
|Class 6||Two-state systems: from ion channels to cooperative binding. (Nobuhiro Hayashi)||Applying statistical mechanics to bio macromolecules expressing as two states.|
|Class 7||Random Walks: A random walk model of polymers viewed as rigid segments connected by hinges, and the basics of structures of macroomolecules such as proteins, DNA/RNA and chromosomes.(Makio Tokunaga)||Understand the nature and basics of structures of biologycal macromolecues as random processes.|
|Class 8||Random Walks and the structures of macromolecules: Force spectroscopy measured by single molecule techniques and explanation by random walks. (Makio Tokunaga)||Understand the various structures of biological macromolecues viewed as the random walk model.|
|Class 9||Beam theory: Architecture for cells and skeletons --- Elasticity, stiffness, persistance length and enrtropy viewed as beam deformations result in stretching, bending.(Makio Tokunaga)||Understand the architecture of biological macromolecules and assemblies viewed as elasticity and thermodynamics.|
|Class 10||Structure and function of cell membrane and membrane proteins.(Satoshi Murakami)||Understanding of physicochemical features of cell membranes and membrane proteins.|
|Class 11||Physioogical aspects on membrane proteins. (Satoshi Murakami)||Understanding of physiological aspects on channels, pumps, transporters and receptors.|
|Class 12||Action potential in neurons and Hodgkin–Huxley model. (Satoshi Murakami)||Understanding of basic concept of Hodgikin-Huxley model|
|Class 13||Rate Equations and Dynamics in the Cell. (Hideki Taguchi)||Describe dynamics of proteins in the cell using rate equations.|
|Class 14||Dynamics of Molecular Motors: Translational motor proteins. (Hideki Taguchi)||Explain the molecular mechanism of translational motor proteins.|
|Class 15||Dynamics of Molecular Motors: Rotary motor proteins. (Hideki Taguchi)||Explain the molecular mechanism of rotary motor proteins.|
“Physical Biology of the Cell, 2nd ed.” Phillips et al, Garland Science, 2012
P. Atkins and J. D. Paula, Physical Chemistry for the Life Science, second edition、Oxford University Press.: P. Atkins and J. D. Paula, Physical Chemistry, eight edition, Oxford University Press. ; ,Student's Solutions Manual to Accompany Atkins' Physical Chemistry, eight edition, Oxford University Press.
Reports and/or presentations on demand to check the essential understanding and quantitative discussion are held for the assessment.
No prerequisites are necessary, but it is highly suggested that the courses of physical chemistry I, II and biochemistry (and/or protein science) are completed prior to attempting this course.
Nobuhiro Hayashi (nhayashi[at]bio.titech.ac.jp, 03-5734-3863) Yoshitaka Ishii (ishii[at]bio.titech.ac.jp, 045-924-5817) Makio Tokunaga (mtoku[at]bio.titech.ac.jp, 045-924-5711) Satoshi Murakami (murakami[at]bio.titech.ac.jp, 045-924-5748) Hideki Taguchi (taguchi[at]bio.titeich.ac.jp, 045-924-5785)