Genetic manipulation techniques have enabled us to modify two major biomolecules, DNA and proteins, artificially. Nowadays, significant modifications of functions of proteins and cells have been achieved by complicated and large-scale modification of DNA/chromosomes based on advanced genetic manipulation technology, thus we can now apply the well modified functions in several applications such as molecular recognition, production of useful compounds, imaging, and so on.
This course covers the bases of protein engineering, genetic engineering, metabolic engineering, chromosome engineering and bioimaging, as well as advanced knowledge in these topics.
By the end of this course, students will be able to:
1) Understand the outline of genetic engineering and metabolic engineering, and acquire the advanced knowledge about genetic and metabolic manipulation of microbes for production of useful compounds.
2) Understand the outline of protein engineering, and acquire the advanced knowledge about protein thermostabilization, molecular evolutional methods, and antibody engineering.
3) Understand the outline of chromosome engineering, and acquire the advanced knowledge about artificial chromosomes, applications in generation of genetically modified organisms, and bioimaging.
Protein engineering, Genetic engineering, Metabolic engineering, Synthetic biology, Chromosome engineering, Bioimaging
|Intercultural skills||Communication skills||Specialist skills||Critical thinking skills||Practical and/or problem-solving skills|
Three instructors provide 5 classes with respect to the selected field in biomolecular engineering by using PowerPoint slides.
|Course schedule||Required learning|
|Class 1||Genetic Engineering: (2) Basic and advanced techniques for PCR||Explain the basis and advanced techniques of PCR essential for recent genetic engineering.|
|Class 2||Genetic Engineering: (2) Applications of PCR techniques||Explain the applications of PCR techniques for recent genetic engineering.|
|Class 3||Metabolic Engineering Overview||Explain the notion and methodologies used for metabolic engineering.|
|Class 4||Metabolic Engineering for biofuel production||Explain how to engineer metabolisms of microbes for production of biofules.|
|Class 5||Metabolic Engineering for biochemical production||Explain how to engineer metabolisms of microbes for production of chemicals.|
|Class 6||Protein Engineering Overview||Explain the notion of and the methodologies used for protein engineering.|
|Class 7||Protein Engineering Applications: (1) Thermostabilization||Explain the significance and useful methodologies for protein thermostabilization as an important application of protein engineering.|
|Class 8||Protein Engineering Applications (2) Rational design||Explain the basis and applications of rational design methods, as an important methodology of protein engineering.|
|Class 9||Protein Engineering Applications (3) Molecular evolution||Explain the basis and several applications of antibody engineering, one of most industrially important area of protein engineering.|
|Class 10||Protein Engineering Applications (4) Antibody Engineering||Explain the basis and several applications of antibody engineering, one of most industrially important area of protein engineering.|
|Class 11||Introduction to artificial chromosomes||Understand the fundamentals and applications of artificial chromosomes.|
|Class 12||Artificial chromosomes and chromosome engineering||Understand genetic engineering using artificial chromosomes, and introduce up-to-date technology of chromosome engineering.|
|Class 13||Genetic and developmental engineering to generate genetically modified organisms||Introduce up-to-date technology in genetic and developmental engineering.|
|Class 14||Bioimaging using genetic engineering techniques||Understand bioimaging techniques using genetically modified proteins, and introduce its applications in recent researches.|
|Class 15||Genome Engineering||Introduction of genome engineering|
Handouts will be distributed at the beginning of class when necessary. The PowerPoint documents that are to be used in the class will be made available in advance via the OCW-i system, as possible.
Students will be assessed by reports indicated by each instructor (25% each).