Genetic manipulation techniques have enabled us to modify two major biomolecules, DNA and proteins, artificially. Nowadays, significant modifications of functions of proteins and cells have been achieved by complicated and large-scale modification of DNA/chromosomes based on advanced genetic manipulation technology, thus we can now apply the well modified functions in several applications such as molecular recognition, production of useful compounds, imaging, and so on.
This course covers the bases of protein engineering, metabolic engineering, artificial genetic circuit, and chromosome engineering, as well as advanced knowledge in these topics.
By the end of this course, students will be able to:
1) Understand the outline of protein engineering, and acquire the advanced knowledge about protein thermostabilization, molecular evolutional methods, and antibody engineering.
2) Understand the outline of metabolic engineering, and acquire the advanced knowledge about metabolic engineering of microbes for production of useful compounds.
3) Understand the outline of artificial genetic circuit, and acquire the advanced knowledge about engineering of genetic code, in vitro synthetic biomolecular system, and application of artificial genetic circuit in metabolic engineering.
4) Understand the outline of chromosome engineering, and acquire the advanced knowledge about artificial chromosomes, applications in generation of genetically modified organisms, and bioimaging.
Protein engineering, Metabolic engineering, Artificial genetic circuit, Synthetic biology, Chromosome engineering, Bioimaging
|Intercultural skills||Communication skills||Specialist skills||Critical thinking skills||Practical and/or problem-solving skills|
Four instructors provide 3-4 classes with respect to the selected field in biomolecular engineering by using PowerPoint slides.
|Course schedule||Required learning|
|Class 1||Protein Engineering Overview||Explain the notion of and the methodologies used for protein engineering.|
|Class 2||Protein Engineering Applications: (1) Thermostabilization||Explain the significance and useful methodologies for protein thermostabilization as an important application of protein engineering.|
|Class 3||Protein Engineering Applications (2) Molecular evolution||Explain the basis and applications of molecular evolutional methods, as an important methodology of protein engineering.|
|Class 4||Protein Engineering Applications (3) Antibody Engineering||Explain the basis and several applications of antibody engineering, one of most industrially important area of protein engineering.|
|Class 5||Metabolic Engineering Overview||Explain the notion of and the methodologies used for metabolic engineering.|
|Class 6||Metabolic Engineering for biofuel production||Explain how to engineer metabolisms of microbes for production of biofules.|
|Class 7||Metabolic Engineering for biochemical production||Explain how to engineer metabolisms of microbes for production of chemicals.|
|Class 8||Engineering of genetic code||Explain mechanism of genetic code, how to modify the mechanism, and how to apply the modification in protein engineering.|
|Class 9||In vitro synthetic biomolecular system||Explain artificial biomolecules such as aptamer and aptazyme, and how to combine them with proteins to construct in vitro artificial life.|
|Class 10||Artificial genetic circuit for metabolic engineering: (1) Mathematical modeling||Explain the basis of mathematical modeling used in artificial genetic circuit in synthetic biology field|
|Class 11||Artificial genetic circuit for metabolic engineering: (2) Applications in metabolic engineering||Explain combination and engineering of proteins used in artificial genetic circuit for metabolic engineering in synthetic biology field|
|Class 12||Introduction to artificial chromosomes||Understand the fundamentals and applications of artificial chromosomes.|
|Class 13||Artificial chromosomes and chromosome engineering||Understand genetic engineering using artificial chromosomes, and introduce up-to-date technology of chromosome engineering.|
|Class 14||Genetic and developmental engineering to generate genetically modified organisms||Introduce up-to-date technology in genetic and developmental engineering, including genome editing.|
|Class 15||Bioimaging using genetic engineering techniques||Understand bioimaging techniques using genetically modified proteins, and introduce its applications in recent researches.|
Handouts will be distributed at the beginning of class when necessary. The PowerPoint documents that are to be used in the class will be made available in advance via the OCW-i system, as possible.
Students will be assessed by reports indicated by each instructor (25% each).