2019 Molecular and Cellular Biology

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Academic unit or major
Graduate major in Life Science and Technology
Instructor(s)
Kimura Hiroshi  Iwasaki Hiroshi  Yamaguchi Yuki  Wakabayashi Ken-Ichi  Aizawa Yasunori 
Course component(s)
Lecture
Day/Period(Room No.)
Tue1-2(J221,S222)  Fri1-2(J221,S222)  
Group
-
Course number
LST.A401
Credits
2
Academic year
2019
Offered quarter
1Q
Syllabus updated
2019/3/18
Lecture notes updated
-
Language used
English
Access Index

Course description and aims

The course will cover the mechanism and regulation of genome expression and dynamics in the context of chromatin and nuclear structure at cellular levels, toward understanding biological phenomena such as development, differentiation, and diseases. Topics will include the structure and function of cell nucleus, chromatin and chromosomes, the organization of genome, the molecular mechanism of transcription, DNA replication, repair, and recombination, and the regulation of the cell cycle and cell motility.

The main goal of the course is to understand the molecular mechanism of genome and chromatin function, which is essential for further learning of higher-order biological function and application for bioengineering.

This is an intermediate to advanced course in molecular and cellular biology. Students are expected to have basic knowledge in Molecular Biology and Cell Biology, and advised to enroll the related basic courses (i.e., Molecular Biology I and II and Biochemistry I and II) in order to receive optimal instruction.

Student learning outcomes

By the end of this course, students will be able to:
1. explain the function and structure of cell nucleus, chromatin, and chromosomes
2. explain the detailed mechanism of eukaryotic transcription
3. explain the genome dynamics and chromatin domains
4. explain the detailed mechanism of eukaryotic DNA replication, repair, and recombination
5. explain the regulatory mechanism and diversity of cell cycle, and the mechanism of cell growth, differentiation, and motility

Keywords

cell nucleus, chromatin, chromosome, transcriptional regulation, genome, DNA transaction, cell cycle, cell growth, cell motility

Competencies that will be developed

Intercultural skills Communication skills Specialist skills Critical thinking skills Practical and/or problem-solving skills
- - -

Class flow

Lectures will be given exclusively in English and no Japanese instruction will be presented. When necessary, in the first 10-15 min of each lecture, a summary of the previous lecture is given, followed by the main points of the day's lecture. In the last 10-15 min of each lecture, a quiz may be given to find out if students have learned the material given. When necessary, a lecture may be given as an exercise style.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Structure and function of cell nucleus, chromatin, and chromosome: (1) The structure Students must be able to explain the structure of cell nucleus, chromatin, and chromosomes (Kimura)
Class 2 Structure and function of cell nucleus, chromatin, and chromosome: (2) The function Students must be able to explain the function of cell nucleus, chromatin, and chromosomes (Kimura)
Class 3 Structure and function of cell nucleus, chromatin, and chromosome: (3) Chromatin and epigenetics Students must be able to explain examples of epigenetics phenomenon and underlying chromatin regulation (Kimura)
Class 4 DNA transaction: (1) Molecular mechanism of eukaryotic DNA replication Students must be able to explain the detailed molecular mechanism of eukaryotic DNA replication (Iwasaki)
Class 5 DNA transaction: (2) DNA damage repair and disease Students must be able to explain the detailed molecular mechanism of eukaryotic DNA repair (Iwasaki)
Class 6 DNA transaction: (3) Molecular mechanism of DNA recombination and meiosis Students must be able to explain the detailed molecular mechanism and biological significance of eukaryotic DNA recombination (Iwasaki)
Class 7 Functional genome and chromatin: (1) The structure and dynamics Students must be able to explain the details of genome structure and dynamics, including transposon, non-coding DNA and non-coding RNA (Aizawa)
Class 8 Functional genome and chromatin: (2) Heterochromatin, euchromatin, and inter-chromatin interaction Students must be able to explain heterochromatin, euchromatin and inter-chromatin interaction (Aizawa)
Class 9 Functional genome: (3) Centromere, telomere, and nuclear positioning Students must be able to explain the nuclear positioning of chromatin, and functional domains like centromeres and telomeres (Aizawa)
Class 10 Regulation of transcription: (1) Molecular mechanism of the initiation, elongation and termination Students must be able to explain the detailed molecular mechanism of the initiation, elongation, and termination of eukaryotic transcription (Yamaguchi)
Class 11 Regulation of transcription: (2) Molecular mechanism of gene repression and activation Students must be able to explain the detailed molecular mechanism of gene repression in eukaryotes (Yamaguchi)
Class 12 Regulation of transcription: (3) Coupling mechanism of transcription with other processes Students must be able to explain the coupling mechanism of transcription with other processes in eukaryotes (Yamaguchi)
Class 13 Cell cycle, growth, and motility: (1) Regulation and diversity of cell cycle Students must be able to explain the regulation and diversity of the eukaryotic cell cycle (Wakabayashi)
Class 14 Cell cycle, growth, and motility: (2) Regulatory mechanism of cell growth and differentiation Students must be able explain the regulatory mechanism of cell growth and differentiation (Wakabayashi)
Class 15 Cell cycle, growth, and motility: (3) Cytoskeleton and molecular motors Students must be able to explain the cytoskeleton and molecular motors (Wakabayashi)

Textbook(s)

none

Reference books, course materials, etc.

none

Assessment criteria and methods

Students will be assessed on their ability to describe the molecular and cellular biology given in the course and to utilize the knowledge for problem solving, and students' course scores are evaluated by students' reports that should be submitted after the course.

Related courses

  • LST.A208 : Molecular Biology I
  • LST.A213 : Molecular Biology II
  • LST.A203 : Biochemistry I
  • LST.A218 : Biochemistry II

Prerequisites (i.e., required knowledge, skills, courses, etc.)

Students are expected to have basic knowledge in Molecular Biology and Cell Biology, and enrollment in Biochemistry I, II and Molecular Biology I, II is encouraged.

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