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- 工学院，物質理工学院，環境・社会理工学院共通科目
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School of Environment and Society
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- Graduate major in Technology and Innovation Management
- Common courses
- Liberal arts and basic science courses
- Academic unit or major
- Undergraduate major in Life Science and Technology
- Instructor(s)
- Seio Kohji Fukui Toshiaki Yamaguchi Yuki Wachi Masaaki Aizawa Yasunori Tagawa Yoh-Ichi Nakatogawa Hitoshi Nonomura Keiko Yatsunami Rie Kajikawa Masaki Kondoh Shinae Yamamoto Naoyuki Kadonosono Tetsuya Tachibana Kazunori Iwasaki Hiroshi Fujita Naonobu Ueda Hiroshi Kitaguchi Tetsuya Yoshida Keisuke Wakabayashi Ken-Ichi Kano Fumi Iwai Noritaka Okumura Eiichi Orita Izumi Kaneko Shinya Sakamoto Satoshi Tanaka Toshiaki Tsubouchi Hideo Fukushima Toshiaki Zhu Bo Nakatsu Daiki Yamamoto Junichi Yasuda Takanobu
- Class Format
- Exercise /Experiment (Face-to-face)
- Media-enhanced courses
- Day/Period(Room No.)
- Mon5-9(緑が丘3号館学生実験室) Thr5-9(緑が丘3号館学生実験室)
- Group
- A
- Course number
- LST.A254
- Credits
- 3
- Academic year
- 2022
- Offered quarter
- 4Q
- Syllabus updated
- 2022/4/20
- Lecture notes updated
- -
- Language used
- Japanese
- Access Index
-
Course description and aims
This course aims at learning principles and methods for basic experiments in molecular biology, which are required for conducting research in the laboratory.
Student learning outcomes
By the end of this course, students will be able to:
1) Conduct basic molecular biological experiments required for research in the laboratory.
2) Solve problems when in trouble with basic molecular biological experiments.
3) Describe the aim, results, and discussion of molecular biological experiments clearly.
Keywords
Molecular biology
Competencies that will be developed
| ✔ Specialist skills | Intercultural skills | ✔ Communication skills | Critical thinking skills | ✔ Practical and/or problem-solving skills |
Class flow
Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class.
Course schedule/Required learning
| Course schedule | Required learning | |
|---|---|---|
| Class 1 | Cultivation of microorganisms 1 | Exercise and experimental report |
| Class 2 | Cultivation of microorganisms 2 | Exercise and experimental report |
| Class 3 | Transformation of Escherichia coli 1 | Exercise and experimental report |
| Class 4 | Transformation of Escherichia coli 2 | Exercise and experimental report |
| Class 5 | Transformation of Escherichia coli 3 | Exercise and experimental report |
| Class 6 | Analysis of DNA topology and higher order structure 1 | Exercise and experimental report |
| Class 7 | Analysis of DNA topology and higher order structure 2 | Exercise and experimental report |
| Class 8 | Analysis of DNA topology and higher order structure 3 | Exercise and experimental report |
| Class 9 | Analysis of mRNA expression 1 | Exercise and experimental report |
| Class 10 | Analysis of mRNA expression 2 | Exercise and experimental report |
| Class 11 | Analysis of mRNA expression 3 | Exercise and experimental report |
| Class 12 | Analysis of gene mutation conferring resistance to antibiotics 1 | Exercise and experimental report |
| Class 13 | Analysis of gene mutation conferring resistance to antibiotics 2 | Exercise and experimental report |
| Class 14 | Analysis of gene mutation conferring resistance to antibiotics 3 | Exercise and experimental report |
Out-of-Class Study Time (Preparation and Review)
To enhance effective learning, students are encouraged to spend a certain length of time outside of class on preparation and review (including for assignments), as specified by the Tokyo Institute of Technology Rules on Undergraduate Learning (東京工業大学学修規程) and the Tokyo Institute of Technology Rules on Graduate Learning (東京工業大学大学院学修規程), for each class.
They should do so by referring to textbooks and other course material.
Textbook(s)
An original textbook edited by the School of Life Science and Technology
Reference books, course materials, etc.
Handouts will be distributed as necessary.
Assessment criteria and methods
Experimental training, report
Related courses
- LST.A208 ： Molecular Biology I
- LST.A213 ： Molecular Biology II
Prerequisites (i.e., required knowledge, skills, courses, etc.)
None
Other
None
