2021 LST Basic Laboratory and Exercise IV C

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Academic unit or major
Undergraduate major in Life Science and Technology
Instructor(s)
Hayashi Nobuhiro  Fukui Toshiaki  Yamaguchi Yuki  Wachi Masaaki  Aizawa Yasunori  Tagawa Yoh-Ichi  Nakatogawa Hitoshi  Yatsunami Rie  Kajikawa Masaki  Kondoh Shinae  Yamamoto Naoyuki  Kadonosono Tetsuya  Tachibana Kazunori  Iwasaki Hiroshi  Fujita Naonobu  Ueda Hiroshi  Kitaguchi Tetsuya  Wakabayashi Ken-Ichi  Kano Fumi  Iwai Noritaka  Okumura Eiichi  Orita Izumi  Kaneko Shinya  Sakamoto Satoshi  Tanaka Toshiaki  Tsubouchi Hideo  Fukushima Toshiaki  Kobayashi Yuki  Zhu Bo  Nakatsu Daiki  Miyanaga Kazuhiko  Yamamoto Junichi 
Course component(s)
Exercise /Experiment    (対面)
Day/Period(Room No.)
Mon5-9(緑が丘3号館学生実験室)  Thr5-9(緑が丘3号館学生実験室)  
Group
C
Course number
LST.A254
Credits
3
Academic year
2021
Offered quarter
2Q
Syllabus updated
2021/3/19
Lecture notes updated
-
Language used
Japanese
Access Index

Course description and aims

This course aims at learning principles and methods for basic experiments in molecular biology, which are required for conducting research in the laboratory.

Student learning outcomes

By the end of this course, students will be able to:
1) Conduct basic molecular biological experiments required for research in the laboratory.
2) Solve problems when in trouble with basic molecular biological experiments.
3) Describe the aim, results, and discussion of molecular biological experiments clearly.

Keywords

Molecular biology

Competencies that will be developed

Specialist skills Intercultural skills Communication skills Critical thinking skills Practical and/or problem-solving skills

Class flow

Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Cultivation of microorganisms 1 Exercise and experimental report
Class 2 Cultivation of microorganisms 2 Exercise and experimental report
Class 3 Transformation of Escherichia coli 1 Exercise and experimental report
Class 4 Transformation of Escherichia coli 2 Exercise and experimental report
Class 5 Transformation of Escherichia coli 3 Exercise and experimental report
Class 6 Analysis of DNA topology and higher order structure 1 Exercise and experimental report
Class 7 Analysis of DNA topology and higher order structure 2 Exercise and experimental report
Class 8 Analysis of DNA topology and higher order structure 3 Exercise and experimental report
Class 9 Analysis of mRNA expression 1 Exercise and experimental report
Class 10 Analysis of mRNA expression 2 Exercise and experimental report
Class 11 Analysis of mRNA expression 3 Exercise and experimental report
Class 12 Analysis of gene mutation conferring resistance to antibiotics 1 Exercise and experimental report
Class 13 Analysis of gene mutation conferring resistance to antibiotics 2 Exercise and experimental report
Class 14 Analysis of gene mutation conferring resistance to antibiotics 3 Exercise and experimental report

Out-of-Class Study Time (Preparation and Review)

To enhance effective learning, students are encouraged to spend a certain length of time outside of class on preparation and review (including for assignments), as specified by the Tokyo Institute of Technology Rules on Undergraduate Learning (東京工業大学学修規程) and the Tokyo Institute of Technology Rules on Graduate Learning (東京工業大学大学院学修規程), for each class.
They should do so by referring to textbooks and other course material.

Textbook(s)

An original textbook edited by the School of Life Science and Technology

Reference books, course materials, etc.

Handouts will be distributed as necessary.

Assessment criteria and methods

Experimental training, report

Related courses

  • LST.A208 : Molecular Biology I
  • LST.A213 : Molecular Biology II

Prerequisites (i.e., required knowledge, skills, courses, etc.)

None

Other

None

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