This course aims at learning principles and methods for basic experiments in molecular biology, which are required for conducting research in the laboratory.
By the end of this course, students will be able to:
1) Conduct basic molecular biological experiments required for research in the laboratory.
2) Solve problems when in trouble with basic molecular biological experiments.
3) Describe the aim, results, and discussion of molecular biological experiments clearly.
|Intercultural skills||✔ Communication skills||✔ Specialist skills||Critical thinking skills||✔ Practical and/or problem-solving skills|
Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class.
|Course schedule||Required learning|
|Class 1||Cultivation of microorganisms 1||Experimental report.|
|Class 2||Cultivation of microorganisms 2||Experimental report.|
|Class 3||Transformation of Escherichia coli 1||Experimental report.|
|Class 4||Transformation of Escherichia coli 2||Experimental report.|
|Class 5||Transformation of Escherichia coli 3||Experimental report.|
|Class 6||Analysis of DNA topology and higher order structure 1||Experimental report.|
|Class 7||Analysis of DNA topology and higher order structure 2||Experimental report.|
|Class 8||Analysis of DNA topology and higher order structure 3||Experimental report.|
|Class 9||Analysis of mRNA expression 1||Experimental report.|
|Class 10||Analysis of mRNA expression 2||Experimental report.|
|Class 11||Analysis of mRNA expression 3||Experimental report.|
|Class 12||Analysis of gene mutation conferring resistance to antibiotics 1||Experimental report.|
|Class 13||Analysis of gene mutation conferring resistance to antibiotics 2||Experimental report.|
|Class 14||Analysis of gene mutation conferring resistance to antibiotics 3||Experimental report.|
An original textbook edited by the School of Life Science and Technology
Handouts will be distributed as necessary.
Experimental training, report