This course aims at learning principles and methods for basic experiments in molecular biology, which are required for conducting research in the laboratory.
By the end of this course, students will be able to:
1) Conduct basic molecular biological experiments required for research in the laboratory.
2) Solve problems when in trouble with basic molecular biological experiments.
3) Describe the aim, results, and discussion of molecular biological experiments clearly.
Molecular biology
✔ Specialist skills | Intercultural skills | ✔ Communication skills | Critical thinking skills | ✔ Practical and/or problem-solving skills |
Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class.
Course schedule | Required learning | |
---|---|---|
Class 1 | Cultivation of microorganisms 1 | Exercise and experimental report |
Class 2 | Cultivation of microorganisms 2 | Exercise and experimental report |
Class 3 | Transformation of Escherichia coli 1 | Exercise and experimental report |
Class 4 | Transformation of Escherichia coli 2 | Exercise and experimental report |
Class 5 | Transformation of Escherichia coli 3 | Exercise and experimental report |
Class 6 | Analysis of DNA topology and higher order structure 1 | Exercise and experimental report |
Class 7 | Analysis of DNA topology and higher order structure 2 | Exercise and experimental report |
Class 8 | Analysis of DNA topology and higher order structure 3 | Exercise and experimental report |
Class 9 | Analysis of mRNA expression 1 | Exercise and experimental report |
Class 10 | Analysis of mRNA expression 2 | Exercise and experimental report |
Class 11 | Analysis of mRNA expression 3 | Exercise and experimental report |
Class 12 | Analysis of gene mutation conferring resistance to antibiotics 1 | Exercise and experimental report |
Class 13 | Analysis of gene mutation conferring resistance to antibiotics 2 | Exercise and experimental report |
Class 14 | Analysis of gene mutation conferring resistance to antibiotics 3 | Exercise and experimental report |
An original textbook edited by the School of Life Science and Technology
Handouts will be distributed as necessary.
Experimental training, report
None
None