2020 LST Basic Laboratory and Exercise III D

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Academic unit or major
Undergraduate major in Life Science and Technology
Ichinose Hiroshi  Ohta Hiroyuki  Kume Shoen  Kawakami Atsushi  Shimojima Mie  Shiraki Nobuaki  Suzuki Takashi  Tanaka Mikiko  Nakamura Nobuhiro  Hirasawa Takashi  Kato Akira  Ogura Shunichiro  Orihara Kanami  Hirota Junji  Masuda Shinji  Kimura Hiroshi  Tanaka Kan  Hisabori Toru  Inohaya Keiji  Sakano Daisuke  Nishihara Hidenori  Hara Satoshi  Hori Koichi  Miyanaga Kazuhiko  Chen Xinyue  Nagashima Ayumi  Sato Yuko  Kobayashi Yuki  Yoshida Keisuke  Hayashi Nobuhiro 
Course component(s)
Exercise /Experiment
Mode of instruction
Day/Period(Room No.)
Intensive (緑が丘3号館学生実験室)  
Course number
Academic year
Offered quarter
Syllabus updated
Lecture notes updated
Language used
Access Index

Course description and aims

This course aims at learning principles and methods for basic experiments in biochemistry, which are required for conducting research in the laboratory.

Student learning outcomes

By the end of this course, students will be able to:
1) Conduct basic biochemical experiments required for research in the laboratory.
2) Solve problems when in trouble with basic biochemical experiments.
3) Describe the aim, results, and discussion of biochemical experiments clearly.



Competencies that will be developed

Specialist skills Intercultural skills Communication skills Critical thinking skills Practical and/or problem-solving skills

Class flow

Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Basic biochemical experiments 1: preparation of reagents, use of pH meter Exercise and experimental report
Class 2 Basic biochemical experiments 2: centrifugation Exercise and experimental report
Class 3 Protein experiments 1: extraction, separation, and quantification Exercise and experimental report
Class 4 Protein experiments 2: electrophoresis, CBB stain, and electrotransfer to nylon membrane Exercise and experimental report
Class 5 Protein experiments 3: western blotting and immunostaining Exercise and experimental report
Class 6 Analysis of lipids and organelles 1: extraction and separation of lipids from plants Exercise and experimental report
Class 7 Analysis of lipids and organelles 2: photosynthetic reaction of chloroplasts Exercise and experimental report
Class 8 Analysis of lipids and organelles 3: isolation and functional analysis of mitochondria Exercise and experimental report
Class 9 Microorganisms and phages 1: phage quantification Exercise and experimental report
Class 10 Microorganisms and phages 2: bacterial phage infection and Gram staining Exercise and experimental report
Class 11 Light microscopy of culture cells 1: phase-contrast microscopy of cell division, fluorescence staining Exercise and experimental report
Class 12 Light microscopy of culture cells 2: fluorescence microscopy of cell division, cell death, and organelles Exercise and experimental report
Class 13 Animal development 1: early development Exercise and experimental report
Class 14 Animal development 2: cell differentiation and organ formation Exercise and experimental report


An original textbook edited by the School of Life Science and Technology

Reference books, course materials, etc.

Handouts will be distributed as necessary.

Assessment criteria and methods

Experimental training, report, exercise

Related courses

  • LST.A203 : Biochemistry I
  • LST.A218 : Biochemistry II
  • LST.A251 : LST Basic Laboratory and Exercise I
  • LST.A252 : LST Basic Laboratory and Exercise II
  • LST.A254 : LST Basic Laboratory and Exercise IV

Prerequisites (i.e., required knowledge, skills, courses, etc.)


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