2020 LST Basic Laboratory and Exercise I D

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Academic unit or major
Undergraduate major in Life Science and Technology
Ishii Yoshitaka  Itoh Takehiko  Ueno Takafumi  Kamachi Toshiaki  Kobatake Eiry  Kitao Akio  Tokunaga Makio  Murakami Satoshi  Osada Toshiya  Hayashi Nobuhiro  Mie Masayasu  Miyashita Eizo  Yamada Takuji  Asakura Noriyuki  Taguchi Hideki  Fujii Masaaki  Akama Hiroyuki  Ishiuchi Shun-Ichi  Abe Satoshi  Ito Yuma  Okada Ui  Kajitani Rei  Kamihara Takayuki  Tran Duy Phuoc  Denda Kimitoshi  Furuta Tadaomi  Mashimo Yasumasa  Niwa Tatsuya  Hirata Keisuke  Ito Hidehiro 
Class Format
Exercise /Experiment     
Media-enhanced courses
Day/Period(Room No.)
Intensive 2-8(緑が丘3号館学生実験室)  
Course number
Academic year
Offered quarter
Syllabus updated
Lecture notes updated
Language used
Access Index

Course description and aims

This course aims at learning the experimental principles and methods of physical chemistry and related knowledge that are required to carry out bachelor thesis research.

Student learning outcomes

By the end of this course, students will be able to:
1) Conduct basic experiments required for bachelor thesis research.
2) Devise a troubleshooting method when the experiment fails.
3) Clearly note the purpose, results, and discussion of the experiment.


Physical chemistry

Competencies that will be developed

Specialist skills Intercultural skills Communication skills Critical thinking skills Practical and/or problem-solving skills

Class flow

The experiment will be conducted after explaining the experiment in about 30 minutes.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Basics and usage of spectrophotometer I  
Class 2 Basics and usage of spectrophotometer II
Class 3 Separation and purification of proteins I    
Class 4 Separation and purification of proteins II  
Class 5 Separation and purification of proteins III  
Class 6 Kinetic analysis of enzyme reaction I  
Class 7 Kinetic analysis of enzyme reaction II  
Class 8 Kinetic analysis of enzyme reaction III  
Class 9 Analysis of protein-ligand binding by equilibrium dialysis  
Class 10 Measurement of acid dissociation constant  
Class 11 Quantification of diffusion coefficient and Avogadro number by Brownian motion measurement  
Class 12 Computer analysis of biomolecules I  
Class 13 Computer analysis of biomolecules II  
Class 14 Computer analysis of biomolecules III  


Original text created by the the School of Life Science and Technology

Reference books, course materials, etc.

No reference book is specified.
Handouts will be distributed as needed.

Assessment criteria and methods

The grade is evaluated by the attitude toward the experiment, the degree of skill of the experiment operation technique, and the experiment report.

Related courses

  • LST.A222 : LST Basic Laboratory II
  • LST.A223 : LST Basic Laboratory III
  • LST.A224 : LST Basic Laboratory IV

Prerequisites (i.e., required knowledge, skills, courses, etc.)

No special requirements

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