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2019　Cell Engineering

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Academic unit or major
Undergraduate major in Life Science and Technology
Instructor(s)
Kume Shoen  Hirota Junji  Tagawa Yoh-Ichi 
Class Format
Lecture     
Media-enhanced courses
Day/Period(Room No.)
Tue7-8(H114)  Fri7-8(H114)  
Group
-
Course number
LST.A352
Credits
2
Academic year
2019
Offered quarter
2Q
Syllabus updated
2019/4/4
Lecture notes updated
-
Language used
Japanese
Access Index

Course description and aims

Cell engineering involves the characterization and manipulation of cell functions with their application in life science research and regenerative medicine.The couse will cover the base and aplicaitons of celll engineering, combined with molecular cell biology, developmental biology and genetic engineering to establish fundamental understanting of property-function relationships and manipulation of cell properties to alter, maintain,restore or improve cell functions. This course will also give an overview of milestones of in vivo genetic engineering and stem cell researchs and expose students to current topics of these fields, in which techniques for cell engineering, live-imaging, gene-targeting, and stem cells engineering will be highlighted.

Student learning outcomes

By the end of this course, students will be able to:
1) Understand how to ulitize cell and cell engineering technology.
2) Understand the principles and applications of cell fusion
3) Understand the principles and applications of transfection.
4) Understand the principles and applications of bioimaging techniques.
5) Establishes a solid base of generation of generically modified animals.
6) Understand stem cell biology including ES and iPS cells.
7) Uderstand the principles and applicaiton of stem cell for regenerative medicine

Keywords

Cell, Cell death, Proliferation, Aging, Immortalization, Cell fusion, gene expression, transfection, bioimaging, transgenic, gene-targeting stem cell, embryonic stem cell, iPS, regenerative medicine

Competencies that will be developed

✔ Specialist skills ✔ Intercultural skills Communication skills Critical thinking skills Practical and/or problem-solving skills

Class flow

In the first quater of the class, a summary of the previous lecture followed by the main points of the day’s lecture posed asquestions are given. Then, these main points are discussed in detail. Always check the required learning for each class and besure to complete them as part of preparation and review.

Course schedule/Required learning

  Course schedule Required learning
Class 1 Introduction to Cell Engineering 1 Understand aims of this course. Stucent will be introduced to cell engineering and regenerative medicine.
Class 2 Introduction to Cell Engineering 2 Understand history and fundamantals of cell engineering, cell sources and stem cells.
Class 3 Cell fusion Understand principles of cell fusion and its application including production of a monoclonal antiboddy.
Class 4 Genetic manipulation of mammalian cells Students will be introduced to genetic manipulation of mammalian cells including designs of expression vector and various techniques of transfection.
Class 5 Bioimaging Understand principles and application of live imaging technology using genetically engineered fluorescent proteins.
Class 6 Introduction to stem cells Students will be provided with an overview of embryogenesis and embryonic stem cell.
Class 7 Transgenesis Understand principles and applications of generation of genetically engineered animals
Class 8 Principle of Gene-targeting Understand principles of gene-targeted animals, including knock-out / knock-in mice.
Class 9 Application of gene-targeting techniques Understand applications of conditional gene-targeting techniques of including Cre-loxP system, drug-induced gene egineering.
Class 10 Gene therapy Understand principles and applicatinos of gene therapy.
Class 11 Stem cell biology and its appliaction Students will be provided with an overview of pluripotent stem cells from embryonic stem cells to iPS cells.
Class 12 Approaches for developmental biology Understand genetic engineering and reprograming of stem cells and iPS cells.
Class 13 in vitro differentiation of iPS cells Understand base of cell differentiation and in vitro differentiation of iPS cells.
Class 14 Cell transplantation and regenerative medicine Students will be provided with an overview of current stem cell applications in regenerative medicine.
Class 15 Modeling disease using iPS cells Understand use of iPS and adult stem cells in modeling desease.

Textbook(s)

None is required.

Reference books, course materials, etc.

Molecular Biology of the Cell, 6th Edition (Bruce Alberts et al., Garland Science)
Biochemistry, 4th Edition (Donald Voet, Judith G. Voet, Wiley)

Assessment criteria and methods

Assessment is based on the final exam (100%).

Related courses

  • LST.A203 ： Biochemistry I
  • LST.A218 ： Biochemistry II
  • LST.A208 ： Molecular Biology I
  • LST.A213 ： Molecular Biology II
  • LST.A406 ： Molecular Developmental Biology and Evolution
  • LST.A336 ： Genetic Engineering
  • LST.A342 ： Biomedical Materials

Prerequisites (i.e., required knowledge, skills, courses, etc.)

Knowledge of Biochemistry, Molecular Biology, Genetic Engineering is required.

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