This course provides a comprehensive view of microbiology, including the history of microbiology, handlings of microorganisms (isolation, culture and observation of microorganisms), taxonomy, cell growth, structures of microbial cells (Bacteria, Archaea, Eukaryotic microbes), energy metabolism, cellular metabolism, pathogenic microbes, metagenome analaysis, symbiosis, and application of microorganisms.
By the end of this course, students will understand;
1) History of microbiology
2) Handlings of microorganisms (Isolation, culture and observation of microorganisms)
3) Taxonomy (Molecular phylogenetics with 16S rRNA)
4) Mathematical expression of growth of microorganisms
5) Structures of microbial cells (Bacteria, Archaea, Eukaryotic microbes)
6) Energy metabolism of microorganisms
7) Cellular metabolism of microorganisms
8) Pathogenic microbes
9) Metagenome analysis
11) Application of microbes
Molecular phylogenetics, Phylogenetic tree, Bacteria, Archaea, Eukaryotic microbes, Metabolism, Respiration, Fermentation, Pathogenic microbes, Metagenome, Symbiosis
|Intercultural skills||Communication skills||Specialist skills||Critical thinking skills||Practical and/or problem-solving skills|
In the first half of class, a summary of the previous lecture is given. In the second half, the main points of the day’s lecture are discussed in detail. Students are asked to check the required learning for each class and be sure to read the related section of the text book as part of preparation and review.
|Course schedule||Required learning|
|Class 1||History of microbiology||Explain about Louis Pasteur, Robert Koch, and Antonie van Leeuwenhoek|
|Class 2||Handlings of microorganisms (Isolation, culture and obserbation of microorganisms)||Understand handing of microorganisms|
|Class 3||Taxosonomy (Evolution and molecular phylogenetics)||Understand molecular phylogenetics|
|Class 4||Structures of microorganims (1): Bacteria, archaea, and virus||Understand cell structures of Gram-positive and Gram-negative bacteria|
|Class 5||Structures of microorganisms (2): Eukaryotic microbes (Yeast, fungi, and protists)||Understand structures of eukaryoteic cells|
|Class 6||Energy metabolism of microorganisms (1): Glycolysis and fermentation||Understand glycolysis|
|Class 7||Energy metabolism of microorganisms (2): Respiration and photosynthesis||Understand TCA cycle, electron transport chain, and photosynthesis|
|Class 8||Cellular metabolism: Amino acids, nucleic acids, and so on||Understand nitrogen metabolism|
|Class 9||Microbial growth: Mathematical expressin of growth of microorganisms, celldivision, and factors affecting cell growth||Understand growth rate constant and continuous culture|
|Class 10||Microbial genetics: Transformation, trunsduction and recombination||Understand DNA and gene|
|Class 11||Pathogenic microbes||Understand bacterial virulence|
|Class 12||Metagenome analysis||Understand metagenome and its application|
|Class 13||Symbiosis||Understand symbiotic intestinal microflora|
|Class 14||Application of microbes (1）||Understand application of microbes for food industry|
|Class 15||Application of microbes (2）||Understand application of microbes for health industry|
Handouts will be distributed at the beginning of class when necessary and elaborated on using PowerPoint slides.
Brock Biology of Microorganisms (14th edition)
No prerequisites are necessary, but enrollment in the related courses is desirable except Environmental Microbiology (LST.A503).
Masaaki Wachi (mwachi[at]bio.titech.ac.jp, 5770), Takashi Hirasawa (thirasawa[at]bio.titech.ac.jp, 5780), Naoyuki Yamamoto（n-yamamoto[at]bio.titech.ac.jp、5105）
Students may approach the instructors at the end of class or visit their offices upon securing an appointment through e-mail.