How the green fluorescence protein (GFP) emits light is discussed from various perspectives such as physical chemistry, organic chemistry, and biochemistry to introduce the idea that structure makes function. Through active learning with the use of molecular models and tablet PCs, students are guided to an intuitive understanding of the flow of genetic information (the central dogma), protein structure and folding, the mechanism by which chromophore emits fluorescence, the technology behind fluorescence microscopy and so on, leading to a higher level of interest in life science and technology.
Since this course is closely related to Frontiers of Science and Technology, students are advised to enroll these courses continuously.
By the end of this course, students will be able to:
1. understand how GFP emits light from various perspectives such as physical chemistry, organic chemistry, biochemistry, and structural biology.
2. understand, at the basic level, and explain the structure of GFP that is critical for emitting light, the flow of genetic information that makes protein, and technology behind fluorescence microscopy.
Green fluorescent protein (GFP), the central dogma, protein folding, fluorescence microscopy
|Intercultural skills||Communication skills||Specialist skills||Critical thinking skills||Practical and/or problem-solving skills|
Both lecture and exercise are conducted in parallel. The equipment of the Lecture Theater (W531) is utilized. Students are asked to solve problems using molecular models, tablet PCs, and other tools in the course.
|Course schedule||Required learning|
|Class 1||How GFP emits light #1: With a focus on various organic molecules found in nature, the mechanism of fluorescence emission is explained at the atomic level.||Students must be able to explain the physicochemical mechanism by which various organic molecules emit light.|
|Class 2||How GFP emits light #2: The physicochemical properties of p-hydroxybenzylidene imidazolinone, the chromophore of GFP, are explained in order to show exactly how GFP emits fluorescence.||Students must be able to describe the molecular structure of the chromophore of GFP and to explain the mechanism of fluorescence emission in detail.|
|Class 3||rotein structure underlying fluorescence emission of GFP #1: The entire molecular structure of GFP is examined at the atomic level from the perspective of structural biology.||Students must be able to describe the characteristics of the tertiary structure of GFP.|
|Class 4||Protein structure underlying fluorescence emission of GFP #2: The process of protein folding, which allows the formation of a specific tertiary structure from its linear sequence, is explained using GFP as an example.||Students must be able to describe the process of protein folding using GFP as an example.|
|Class 5||The process leading to the production of GFP in cells #1: How the genetic information for GFP is securely stored in the cell nucleus and maintained over generations will be explained.||Student must be able to explain the structure of DNA double helix at the atomic level and the molecular basis for its maintenance over generations.|
|Class 6||The technological basis for fluorescence microscopy: The technological basis for the observation of fluorescence emitted from GFP and its variants is explained.||Students must be able to explain the basic mechanism for fluorescence microscopy.|
|Class 7||Cell biological study using GFP: By using GFP, the pattern of gene expression, subcellular localization of a protein and biochemical processes in the cell can be visualized. Various applications of GFP are presented.||Students must be able to raise some examples on cell biological study using GFP.|
Assessment is based on the quality of in-class assignments. Full attendance is compulsory.
There are no prerequisites, but it is recommended that students have successfully completed Frontiers of Science and Technology.